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Journal / Immunology and Infection / Kontaktfri samodlingsmodell för studier av…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Kontaktfri samodlingsmodell för studier av medfödd immuncellsaktivering under luftvägsvirusinfektion
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Kontaktfri samodlingsmodell för studier av medfödd immuncellsaktivering under luftvägsvirusinfektion

Article DOI: 10.3791/62115-v • 07:36 min • February 28th, 2021
February 28th, 2021 •
*1, *1, 1, 2, 3, 1,4, 1,4, 5, 2,6, 1,2,6, 1,6
1Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 3Haartman Institute, Medicum, University of Helsinki, 4Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, 5Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), 6NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore
* These authors contributed equally

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Detta protokoll beskriver en undersökning av de tidiga interaktionerna mellan viralt infekterade nasala epitelceller och medfödd cellaktivering. Individuella delmängder av immunceller kan särskiljas baserat på deras aktivering som svar på virusinfektioner. De kan sedan undersökas ytterligare för att bestämma deras effekter på tidiga antivirala svar.

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Immunologi och infektion utgåva 168 Samodling humana nasala epitelceller medfödda immunceller medfödda T-celler andningsvirus influensa cytokiner flödescytometri
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