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Journal / Biochemistry / Plattform-Inkubator mit beweglicher XY-Stufe:…
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Biochemistry

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JoVE Journal Biochemistry
Plattform-Inkubator mit beweglicher XY-Stufe
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Plattform-Inkubator mit beweglicher XY-Stufe: Eine neue Plattform zur Implementierung der in-Cell schnellen photochemischen Oxidation von Proteinen

Article DOI: 10.3791/62153-v • 05:50 min • May 17th, 2021
May 17th, 2021 •
1, 2, 1, 3, 1
1Department of Pharmaceutical Sciences, University of Maryland Baltimore, 2Technology Research Center, University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology, University of Massachusetts

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Eine neue statische Plattform wird verwendet, um Proteinstruktur- und Interaktionsstellen in der nativen Zellumgebung zu charakterisieren, indem eine Protein-Footprinting-Technik verwendet wird, die als schnelle photochemische Oxidation von Proteinen (IC-FPOP) in der Zelle bezeichnet wird.

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Biochemie Ausgabe 171 Schnelle photochemische Oxidation von Proteinen (FPOP) Protein-Fußabdruck Hydroxylradikale Proteom-breite Strukturbiologie Proteomik Massenspektrometrie
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