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JoVE Journal
Biology

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使用肠道器官模型研究猿特异性相互作用的文化方法
 
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使用肠道器官模型研究猿特异性相互作用的文化方法

Article DOI: 10.3791/62330-v 07:49 min March 23rd, 2021
March 23rd, 2021

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这里,我们提出了两个协议,允许肠道特定的相互作用建模。器官衍生肠道单层和空气-液体接口(ALI)培养物有助于产生从亮面和玄武岩两侧获得分化良好的表皮,而极性倒置肠道器官暴露其腹腔侧,并适合高通量检测。

Tags

生物学,第169期,肠道器官,皮库巴细胞极性,肠道单层,细胞培养膜插入,极性反转,模型
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