Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / DNA Tension Probes to Map the Transient…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Bioengineering
DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
Journal (Bioengineering)
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles
129Xe Hyperpolarization and Assessment of Polarization Loss During Transport (Video) | JoVE
Journal (Bioengineering)
129Xe Hyperpolarization and Assessment of Polarization Loss During Transport (Video) | JoVE
 

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Article DOI: 10.3791/62348-v • 06:53 min • March 20th, 2021
March 20th, 2021 •
1, 2, 1, 1, 2, 1,2
1Department of Chemistry, Emory University, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

Chapters

Summary
Automatic Translation

This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time.

Tags

DNA Tension Probes Molecular Forces Immune Cells Mechanotransduction Mechanobiology Fluorescence Microscopy Force Transmission Coverslips Ethanol Water Ultrasonic Cleaner Piranha Solution Etching
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter