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Metodi quantitativi per studiare l'attività delle proteine arginina metiltransferasi 1-9 nelle cellule
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Metodi quantitativi per studiare l'attività delle proteine arginina metiltransferasi 1-9 nelle cellule

Article DOI: 10.3791/62418-v • 08:11 min • August 7th, 2021
August 7th, 2021 •
1, 1, 1,2
1Structural Genomics Consortium, University of Toronto, 2Department of Pharmacology and Toxicology, University of Toronto

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Questi protocolli forniscono la metodologia utilizzata per valutare l'attività enzimatica dei singoli membri della proteina arginina metiltransferasi (PRMT) nelle cellule. Vengono descritte linee guida dettagliate sulla valutazione dell'attività delle PRMT utilizzando biomarcatori endogeni ed esogeni, anticorpi di riconoscimento metil-arginina e composti degli utensili inibitori.

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Biologia numero 174
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