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Developmental Biology

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JoVE Journal Developmental Biology
En forenklet metode til generering af nyreorganoider fra humane pluripotente stamceller
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En forenklet metode til generering af nyreorganoider fra humane pluripotente stamceller

Article DOI: 10.3791/62452-v • 07:39 min • April 13th, 2021
April 13th, 2021 •
1, 1, 2, 2, 2, 1,3
1Department of Developmental Biology, University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland, 3Center for Critical Care Nephrology, University of Pittsburgh, School of Medicine

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Her beskriver vi en protokol til generering af nyreorganoider fra humane pluripotente stamceller (hPSC'er). Denne protokol genererer nyreorganoider inden for to uger. De resulterende nyreorganoider kan dyrkes i store spinnerkolber eller magnetiske omrøringsplader med flere brønde til parallelle lægemiddeltestmetoder.

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Udviklingsbiologi udgave 170 nyreorganoider CHIR99021 knock-out serumerstatning embryoide kroppe nyrevæv pluripotente stamceller suspensionskultur
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