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Journal / Biochemistry / Visualisering og kvantificering af TGFβ/BMP/SMAD…
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Biochemistry

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JoVE Journal Biochemistry
Visualisering og kvantificering af TGFβ/BMP/SMAD Signalering under forskellige væskeforskydningsstress ved hjælp af proximity-ligation-assay
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Visualisering og kvantificering af TGFβ/BMP/SMAD Signalering under forskellige væskeforskydningsstress ved hjælp af proximity-ligation-assay

Article DOI: 10.3791/62608-v • 11:38 min • September 14th, 2021
September 14th, 2021 •
1,2, 1, 1
1Institute for Chemistry and Biochemistry, Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation

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Her etablerer vi en protokol til samtidig at visualisere og analysere flere SMAD-komplekser ved hjælp af nærhed ligation assay (PLA) i endotelceller udsat for patologiske og fysiologiske væskeforskydningsstress betingelser.

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Biokemi Udgave 175 væskeforskydningsstress nærhed ligation assay endotelceller SMADs BMP TGFβ åreforkalkning flow
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