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Biochemistry

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JoVE Journal Biochemistry
Ekstraktion og visualisering af proteinaggregater efter behandling af Escherichia coli med en proteotoxisk stressor
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Ekstraktion og visualisering af proteinaggregater efter behandling af Escherichia coli med en proteotoxisk stressor

Article DOI: 10.3791/62628-v • 07:59 min • June 29th, 2021
June 29th, 2021 •
1, 1, 1, 1
1School of Biological Sciences, Illinois State University

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Denne protokol beskriver ekstraktion og visualisering af aggregerede og opløselige proteiner fra Escherichia coli efter behandling med en proteotoxisk antimikrobiel. Efter denne procedure giver mulighed for en kvalitativ sammenligning af proteinaggregatdannelse in vivo i forskellige bakteriestammer og/eller mellem behandlinger.

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Biokemi Udgave 172 Stressrespons protein aggregering protein udfolder sig bakterier protein kvalitetskontrol oxidativ stress antimikrobielle stoffer proteostase
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