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Journal / Immunology and Infection / 使用多参数光伏平台对钙流入和HIV-1感染进行单细胞特征描述…
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Immunology and Infection

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JoVE Journal Immunology and Infection
使用多参数光伏平台对钙流入和HIV-1感染进行单细胞特征描述
Tuning Degradation to Achieve Specific and Efficient Protein Depletion
Journal (Immunology and Infection)
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Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo
Journal (Immunology and Infection)
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使用多参数光伏平台对钙流入和HIV-1感染进行单细胞特征描述

Article DOI: 10.3791/62632-v • 07:15 min • May 18th, 2021
May 18th, 2021 •
1, 2, 3,4, 2, 1
1Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, 2Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 3Black Family Stem Cell Institute, Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, 4Sema4, a Mount Sinai venture

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在这里，我们提出了一个协议，其中单个细胞被监测急性事件和生产性HIV-1感染的纳米流体设备。成像数据定义病毒-宿主受体相互作用和信号通路动力学。这是研究信号动力学和分子相互作用的纳米流体高通量纵向单细胞培养和成像的第一种方法。

Tags

免疫学和感染，第171期，高全，光伏，活细胞成像，艾滋病毒感染，钙流入，纯素受体
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