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Analisi dello stress ossidativo negli organoidi intestinali murini utilizzando la sonda fluorogenica sensibile alle specie reattive dell'ossigeno
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Analisi dello stress ossidativo negli organoidi intestinali murini utilizzando la sonda fluorogenica sensibile alle specie reattive dell'ossigeno

Article DOI: 10.3791/62880-v • 09:31 min • September 17th, 2021
September 17th, 2021 •
1,3, 1,4, 1,2,5, 1,6
1Molecular Microbial Pathogenesis Unit, Institut Pasteur, 2Chaire de Microbiologie et Maladies Infectieuses, Collège de France, 3Institut de Biologie Paris Seine (IBPS) - Developmental Biology Unit, Sorbonne Université, CNRS UMR7622, INSERM U1156, 4Molecular Radiotherapy, INSERM U1030, Gustave Roussy, Université Paris-Saclay, 5The Center for Microbes, Development and Health, Institut Pasteur Shanghai and Chinese Academy of Sciences, 6Microenvironment and Immunity Unit, Institut Pasteur

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Il presente protocollo descrive un metodo per rilevare le specie reattive dell'ossigeno (ROS) negli organoidi murini intestinali utilizzando l'imaging qualitativo e i saggi quantitativi di citometria. Questo lavoro può essere potenzialmente esteso ad altre sonde fluorescenti per testare l'effetto di composti selezionati sui ROS.

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Biologia Numero 175 Organoidi intestinali murini Rilevazione ROS Analisi citometria a flusso Rilevamento di immagini dal vivo cellule staminali intestinali colorante ROS-sensibile stress ossidativo sonde fluorogeniche
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