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Journal / Neuroscience / Imaging Dell'assorbimento mitocondriale di Ca2+…
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Neuroscience
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JoVE Journal Neuroscience
Imaging Dell'assorbimento mitocondriale di Ca2+ in astrociti e neuroni utilizzando indicatori Ca2+ geneticamente codificati (GECI)
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Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
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Journal (Neuroscience)
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Imaging Dell'assorbimento mitocondriale di Ca2+ in astrociti e neuroni utilizzando indicatori Ca2+ geneticamente codificati (GECI)

Article DOI: 10.3791/62917-v • 07:46 min • January 22nd, 2022
January 22nd, 2022 •
1, 1,2, 2, 1,2
1Dalton Cardiovascular Research Center, University of Missouri-Columbia, 2Department of Biomedical, Biological and Chemical Engineering, University of Missouri-Columbia

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Questo proctocollo mira a fornire un metodo per l'imaging mitocondriale In vitro e in vivo Ca2+ in astrociti e neuroni.

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Neuroscienze Numero 179 astrociti neuroni mitocondri GECI GCaMP5G/6s sequenza di targeting dei mitocondri promotore gfaABC1D promotore CaMKII imaging in vivo a 2 fotoni
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