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JoVE Journal Environment
En standardisert prosedyre for overvåking av skadelige algeoppblomstringer i Chile ved metabarkodingsanalyse
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En standardisert prosedyre for overvåking av skadelige algeoppblomstringer i Chile ved metabarkodingsanalyse

Article DOI: 10.3791/62967-v • 09:47 min • August 26th, 2021
August 26th, 2021 •
1, 1, 2, 3, 3, 3, 4, 5, 6, 6, 4, 4, 7, 5, 3, 1
1Office of Research and Academia-Government-Community Collaboration, Hiroshima University, 2Graduate School of Human and Environmental Studies, Kyoto University, 3Scientific and Biotechnological Bioresource Nucleus, Universidad de La Frontera, 4Centro de Estudios de Algas Nocivas, Instituto de Fomento Pesquero, 5Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, 6Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, 7Japan Fisheries Research and Education Agency, Fisheries Resources Institute

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Denne protokollen introduserer trinn for metabarkodingsanalyse, rettet mot 16S rRNA- og 18S rRNA-gener, for overvåking av skadelige algeoppblomstringer og deres tilhørende mikrobiom i sjøvannsprøver. Det er et kraftig molekylært basert verktøy, men krever flere prosedyrer, som forklares visuelt her trinn for trinn.

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Miljøvitenskap utgave 174 skadelige algeoppblomstringer Chile HAB-overvåking metabarkodingsanalyse algebakteriell gjensidighet planteplankton lakseoppdrett
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