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JoVE Journal Bioengineering
Een high-yield streptomyces transcriptie-vertaling toolkit voor synthetische biologie en natuurlijke producttoepassingen
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Een high-yield streptomyces transcriptie-vertaling toolkit voor synthetische biologie en natuurlijke producttoepassingen

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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Dit protocol beschrijft een verbeterde methode voor het synthetiseren van hoge opbrengsten van recombinante eiwitten uit een Streptomyces venezuelae celvrije transcriptie-translatie (TX-TL) systeem.

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Bioengineering Celvrije eiwitsynthese in vitro transcriptie-translatie Streptomyces synthetische biologie systeembiologie celvrije systemen biosynthese
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