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JoVE Journal Neuroscience
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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Article DOI: 10.3791/63038-v • 09:50 min • December 25th, 2021
December 25th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 2Nanotechnology Research and Application Center (SUNUM), Sabanci University

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Summary
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This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.

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Digital Droplet PCR AAV Transduction Efficiency Murine Retina Quantification Method Sensitivity Absolute Quantification Target DNA Reporter Gene CRISPR-based Constructs Transduction Rate Straight Forward Results Efficiency Droplet PCR Methodology Quantitative Real-time PCR AAV Titrating Mitochondrial DNA Quantification CRISPR-based Mutation
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