Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolering og karakterisering af musens primære…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Isolering og karakterisering af musens primære lever sinusformede endotelceller
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Journal (Biology)
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle
Journal (Biology)
Rodent Estrous Cycle Monitoring Utilizing Vaginal Lavage: No Such Thing As a Normal Cycle
 
Click here for the English version

Isolering og karakterisering af musens primære lever sinusformede endotelceller

Article DOI: 10.3791/63062-v • 08:22 min • December 16th, 2021
December 16th, 2021 •
1, 1, 1, 1,2
1Division of Gastroenterology & Hepatology, Mayo Clinic, 2Division of Pediatric Gastroenterology, Mayo Clinic

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her skitserer og demonstrerer vi en protokol for primær muselever sinusformet endotelcelle (LSEC) isolering. Protokollen er baseret på leverkollagennaseperfusion, ikke-parenkymal cellerensning ved centrifugering ved lavhastighedscentrifugering og CD146 magnetisk perlevalg. Vi fænotype og karakteriserer også disse isolerede LDC'er ved hjælp af flowcytometri og scanningselektronmikroskopi.

Tags

Biologi udgave 178 lever sinusformede endotelceller (LDC'er) kollagenaseperfusion magnetiske perler positiv selektion flowcytometri immunhistokemi scanning elektronmikroskopi
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter