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JoVE Journal Biology
Isolering af primære patientspecifikke aorta-glatte muskelceller og semikvantemittende realtidskontraktionsmålinger in vitro
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Isolering af primære patientspecifikke aorta-glatte muskelceller og semikvantemittende realtidskontraktionsmålinger in vitro

Article DOI: 10.3791/63122-v • 08:28 min • February 15th, 2022
February 15th, 2022 •
*1,2,3, *1,2, 1,2
1Department of Vascular Surgery, Amsterdam UMC, Vrije Universiteit Amsterdam, 2Department of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC, Vrije Universiteit Amsterdam, 3Laboratory of Experimental Cardiology, Department of Cardiology, Leiden University Medical Center
* These authors contributed equally

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Dette papir beskriver en eksplantationskulturbaseret metode til isolering og dyrkning af primære, patientspecifikke humane aorta glatte muskelceller og dermale fibroblaster. Desuden præsenteres en ny metode til måling af cellekontraktion og efterfølgende analyse, som kan bruges til at studere patientspecifikke forskelle i disse celler.

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Biologi udgave 180 Glat muskelcelle aortaaneurisme sammentrækning translationel in vitro sammentrækningsassay patientspecifik primær celleisolering
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