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Brug af cellemetabolisk fluxanalysator i realtid til at overvåge osteoblastbioenergetika
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Brug af cellemetabolisk fluxanalysator i realtid til at overvåge osteoblastbioenergetika

Article DOI: 10.3791/63142-v • 09:43 min • March 1st, 2022
March 1st, 2022 •
1,2, 1,2, 1,2,3
1Vanderbilt Center for Bone Biology, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 3Department of Molecular Physiology and Biophysics, Vanderbilt University

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Real-time cell metabolic flux assay måler iltforbrugshastigheden og ekstracellulær forsuringshastighed, hvilket svarer til mitokondriel og glykolytisk adenosintrifosfatproduktion ved hjælp af pH- og iltsensorer. Manuskriptet forklarer en metode til at forstå osteoblasternes energistatus og karakteriseringen og fortolkningen af den cellulære bioenergetiske status.

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Biologi udgave 181
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