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Konfokal billeddannelse med høj gennemstrømning af kvantepunkt-konjugerede SARS-CoV-2 spike-trimmere til at spore binding og endocytose i HEK293T-celler
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Konfokal billeddannelse med høj gennemstrømning af kvantepunkt-konjugerede SARS-CoV-2 spike-trimmere til at spore binding og endocytose i HEK293T-celler

Article DOI: 10.3791/63202-v • 06:39 min • April 21st, 2022
April 21st, 2022 •
1, 2, 2,3, 2, 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation

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I denne protokol muliggør kvantepunkter konjugeret til rekombinant SARS-CoV-2-spike cellebaserede assays at overvåge spikebinding til hACE2 ved plasmamembranen og efterfølgende endocytose af de bundne proteiner i cytoplasmaet.

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Biologi udgave 182
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