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Journal / Immunology and Infection / Generering av nollmutanter för att belysa rollen…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Generering av nollmutanter för att belysa rollen som bakteriella glykosyltransferaser i bakteriell motilitet
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
Journal (Immunology and Infection)
Analyzing the Permeability of the Blood-Brain Barrier by Microbial Traversal through Microvascular Endothelial Cells
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
Journal (Immunology and Infection)
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
 
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Generering av nollmutanter för att belysa rollen som bakteriella glykosyltransferaser i bakteriell motilitet

Article DOI: 10.3791/63231-v • 12:29 min • March 11th, 2022
March 11th, 2022 •
1, 2,3, 2,4, 1
1Departamento de Genética, Microbiología y Estadística, Universidad de Barcelona, 2Human Health Therapeutics Research Centre, National Research Council Canada, 3Department of Chemistry, Carleton University, 4Department of Biology, Carleton University

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Här beskriver vi konstruktionen av nollmutanter av Aeromonas i specifika glykosyltransferaser eller regioner innehållande glykosyltransferaser, motilitetsanalyserna och flagellarening som utförs för att fastställa involveringen och funktionen hos deras kodade enzymer i biosyntesen av en glykan, liksom rollen för denna glykan i bakteriell patogenes.

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Immunologi och infektion utgåva 181 O-glykosylylylering glykosyltransferaser heteroglykan in-frame mutanter mesofila Aeromonas flagelliner flagellarening CsCl-gradient
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