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Journal / Bioengineering / 巨型单层囊泡内重组细胞骨架的快速封装…
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Bioengineering

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JoVE Journal Bioengineering
巨型单层囊泡内重组细胞骨架的快速封装
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巨型单层囊泡内重组细胞骨架的快速封装

Article DOI: 10.3791/63332-v • 07:48 min • November 10th, 2021
November 10th, 2021 •
*1, *1, 2, 3, 1,4,5,6
1Department of Mechanical Engineering, University of Michigan, Ann Arbor, 2John A. Paulson School of Engineering and Applied Sciences, Harvard University, 3Department of Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, 5Department of Biophysics, University of Michigan, Ann Arbor, 6Cellular and Molecular Biology Program, University of Michigan, Ann Arbor
* These authors contributed equally

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本文介绍了一种简单的方法，用于快速生产具有包封细胞骨架蛋白的巨型单层囊泡。该方法被证明可用于自下而上的细胞骨架结构在约束和细胞骨架 - 膜相互作用中的重建。

Tags

生物工程，第177期，乳液转移，cDICE，GUV，自下而上的重构，肌动蛋白，筋膜
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