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JoVE Journal Cancer Research
Triagem de biblioteca shRNA agrupada para identificar fatores que modulam um fenótipo de resistência a drogas
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Triagem de biblioteca shRNA agrupada para identificar fatores que modulam um fenótipo de resistência a drogas

Article DOI: 10.3791/63383-v • 14:51 min • June 17th, 2022
June 17th, 2022 •
1,2, 1,2, 2,3, 2,3, 1,2,3, 1,2,3
1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

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A triagem de interferência de RNA de alto rendimento (RNAi) usando um pool de shRNAs lentiviral pode ser uma ferramenta para detectar alvos letais sintéticos terapeuticamente relevantes em malignidades. Fornecemos uma abordagem de triagem de shRNA agrupada para investigar os efeitos epigenéticos na leucemia mielóide aguda (LMA).

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Pesquisa do Câncer Questão 184 Triagem de alto rendimento triagem RNAi shRNA fator epigenético cytarabina resistência arac
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