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JoVE Journal Cancer Research
Freeze-Fracture Elektronenmicroscopie voor extracellulaire vesikelanalyse
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Freeze-Fracture Elektronenmicroscopie voor extracellulaire vesikelanalyse

Article DOI: 10.3791/63550-v • 11:30 min • September 16th, 2022
September 16th, 2022 •
1, 1, 1, 1
1Faculty of Medicine, Institute of Cell Biology, University of Ljubljana

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We presenteren een protocol voor de isolatie en bevriezing van extracellulaire blaasjes (EV's) afkomstig van kankerachtige urotheelcellen. De freeze-fracture-techniek onthulde de diameter en vorm van de EV's en - als een uniek kenmerk - de interne organisatie van de EV-membranen. Deze zijn van immens belang om te begrijpen hoe EV's interageren met de membranen van de ontvanger.

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Kankeronderzoek Nummer 187
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