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Journal / Biochemistry / Høyoppløselig nøytronspektroskopi for å studere…
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Biochemistry

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JoVE Journal Biochemistry
Høyoppløselig nøytronspektroskopi for å studere picosekund-nanosekunddynamikk av proteiner og hydreringsvann
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Høyoppløselig nøytronspektroskopi for å studere picosekund-nanosekunddynamikk av proteiner og hydreringsvann

Article DOI: 10.3791/63664-v • 08:48 min • April 28th, 2022
April 28th, 2022 •
1,2, 2, 1,2, 3, 3, 4, 2, 1
1Institut für Angewandte Physik, Universität Tübingen, 2Institut Max von Laue - Paul Langevin (ILL), 3Institut de Biologie Structurale, Université Grenoble Alpes, 4Institut Européen de Chimie et Biologie, Bordeaux INP, Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux

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Nøytron-tilbakespredningsspektroskopi gir en ikke-destruktiv og etikettfri tilgang til ps-ns-dynamikken til proteiner og deres hydreringsvann. Arbeidsflyten presenteres med to studier på amyloidproteiner: på lysozymets tidsoppløste dynamikk under aggregering og på hydreringsvanndynamikken til tau ved fiberdannelse.

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