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Biochemistry

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JoVE Journal Biochemistry
Un approccio basato sulla spettrometria di massa per identificare le fosfatasi delle fosfoproteine e i loro interattori
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Un approccio basato sulla spettrometria di massa per identificare le fosfatasi delle fosfoproteine e i loro interattori

Article DOI: 10.3791/63805-v • 10:17 min • April 29th, 2022
April 29th, 2022 •
1, 1,2
1Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, 2Norris Cotton Cancer Center

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Qui presentiamo un protocollo per l'arricchimento delle fosfatasi fosfoproteine endogene e delle loro proteine interagenti da cellule e tessuti e la loro identificazione e quantificazione mediante proteomica basata sulla spettrometria di massa.

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Biochimica Numero 182
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