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Journal / Bioengineering / Tidsopløst In Vivo-måling af neuropeptiddynamik…
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Bioengineering

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JoVE Journal Bioengineering
Tidsopløst In Vivo-måling af neuropeptiddynamik ved kapacitiv immunoprobe i svinehjerte
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Journal (Bioengineering)
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Tidsopløst In Vivo-måling af neuropeptiddynamik ved kapacitiv immunoprobe i svinehjerte

Article DOI: 10.3791/63926-v • 08:20 min • May 19th, 2022
May 19th, 2022 •
1, 1, 2,3, 1
1Department of Physiology and Biophysics, Case Western Reserve University, 2University of California Los Angeles (UCLA) Cardiac Arrhythmia Center, David Geffen School of Medicine, 3UCLA Neurocardiology Research Program of Excellence, UCLA

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Etablerede immunokemiske metoder til måling af peptidtransmittere in vivo er afhængige af mikrodialyse eller bulkvæskeudtrækning for at opnå prøven til offline analyse. Disse lider dog af spatiotemporale begrænsninger. Den nuværende protokol beskriver fremstilling og anvendelse af en kapacitiv immunoprobe biosensor, der overvinder begrænsningerne i de eksisterende teknikker.

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