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Bestemmelse af bindingsaffinitet (KD) af radioaktivt mærkede antistoffer mod immobiliserede antigener
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Bestemmelse af bindingsaffinitet (KD) af radioaktivt mærkede antistoffer mod immobiliserede antigener

Article DOI: 10.3791/63927-v • 07:39 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 1, 1
1Department of Radiology and Biomedical Imaging, Yale University

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Her beskrives en metode til bestemmelse af bindingsaffinitet (KD) af radioaktivt mærkede antistoffer mod immobiliserede antigener. KD er ligevægtssociationskonstanten, der kan bestemmes ud fra et mætningsbindingseksperiment ved at måle den totale, specifikke og uspecifikke binding af et radioaktivt mærket antistof i forskellige koncentrationer til dets antigen.

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Medicin udgave 184 radioaktivt mærket antistof radiotracer bindende affinitet mætning dissociationskonstant immobiliseret antigen
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