Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / 原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型…
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
Journal (Biology)
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)
Journal (Biology)
One and Two-Step Method of Rat Neutrophil Isolation for Harvesting Neutrophil Extracellular Traps (NETs)
 
Click here for the English version

原代培养中分化的小鼠脂肪细胞：胰岛素抵抗模型

Article DOI: 10.3791/63979-v • 09:48 min • February 17th, 2023
February 17th, 2023 •
1, 1, 1, 1, 1
1Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM)

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该协议描述了从皮下脂肪中分离小鼠前脂肪细胞，它们分化为成熟脂肪细胞以及诱导胰岛素抵抗。通过蛋白质印迹的胰岛素信号通路成员的磷酸化/激活来评估胰岛素作用。该方法可以直接测定原代脂肪细胞的胰岛素抵抗/敏感性。

Tags

生物学，第192期，前脂肪细胞分离，皮下脂肪，胰岛素作用，TNF-α
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter