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Journal / Biochemistry / Peel-blot-teknikken: En…
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Biochemistry

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JoVE Journal Biochemistry
Peel-blot-teknikken
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Journal (Biochemistry)
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Journal (Biochemistry)
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
 
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Peel-blot-teknikken: En Cryo-EM-prøveprepareringsmetode for å skille enkeltlag fra flerlags eller konsentrerte biologiske prøver

Article DOI: 10.3791/64099-v • 07:27 min • June 29th, 2022
June 29th, 2022 •
*1,2, *1, 1,3
1School of Biological Sciences, Georgia Institute of Technology, 2Department of Structural Biology, Genentech, 3School of Chemistry and Biochemistry, Georgia Institute of Technology
* These authors contributed equally

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Peel-blot-teknikken er en cryo-EM-gridpreparasjonsmetode som gjør det mulig å skille flerlags og konsentrerte biologiske prøver i enkeltlag for å redusere tykkelsen, øke prøvekonsentrasjonen og lette bildebehandlingen.

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