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Journal / Genetics / Sviluppo di linee cellulari muscolari knock-out…
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JoVE Journal Genetics
Sviluppo di linee cellulari muscolari knock-out utilizzando l'editing genetico CRISPR/Cas9 mediato da lentivirus
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Sviluppo di linee cellulari muscolari knock-out utilizzando l'editing genetico CRISPR/Cas9 mediato da lentivirus

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Il protocollo descrive come generare mioblasti knock-out utilizzando CRISPR/Cas9, partendo dalla progettazione di RNA guida alla clonazione cellulare e alla caratterizzazione dei cloni knock-out.

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Genetica Numero 184
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