Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Isolation, Characterization, and Therapeutic…
JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
Laboratory Rearing of Stable Flies and Other Muscoid Diptera
Journal (Biology)
Laboratory Rearing of Stable Flies and Other Muscoid Diptera
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
Journal (Biology)
Long-term Culture of Human Breast Cancer Specimens and Their Analysis Using Optical Projection Tomography
 

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022
September 23rd, 2022 •
1,2, 2,3, 2,4, 2,5, 2,5, 2, 2, 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

Chapters

Summary
Automatic Translation

The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

Tags

Isolation Characterization Therapeutic Application Extracellular Vesicles Cultured Human Mesenchymal Stem Cells Differential Centrifugation Translation Of Research Modern Disease Treatments Video Presentation Culture Medium Alpha-MEM Centrifuge Tubes Cell Fragments Cellular Debris Ultracentrifuge Tubes Microvesicle Pellet PBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter