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Isolement, caractérisation et application thérapeutique de vésicules extracellulaires à partir de cellules souches mésenchymateuses humaines en culture
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Isolement, caractérisation et application thérapeutique de vésicules extracellulaires à partir de cellules souches mésenchymateuses humaines en culture

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022
September 23rd, 2022 •
1,2, 2,3, 2,4, 2,5, 2,5, 2, 2, 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

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Le présent protocole décrit la centrifugation différentielle pour isoler et caractériser les VE représentatifs (exosomes et microvésicules) provenant de CSM humaines en culture. D’autres applications de ces véhicules électriques sont également expliquées dans cet article.

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Biologie numéro 187
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