JoVE Journal
Biology
Biology
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Summary
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本文描述了从表达热不稳定猿病毒40和光激活线粒体PhAM切除的转基因小鼠的肾脏中分离条件永生化肾小球内皮细胞的方法。我们描述了使用磁珠、消化步骤、播种和培养 GEC-CD31 阳性从整个肾脏中分离肾小球的程序。