Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

用荧光线粒体分离条件永生化的小鼠肾小球内皮细胞
 
Click here for the English version

用荧光线粒体分离条件永生化的小鼠肾小球内皮细胞

Article DOI: 10.3791/64147-v 11:49 min September 13th, 2022
September 13th, 2022

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

本文描述了从表达热不稳定猿病毒40和光激活线粒体PhAM切除的转基因小鼠的肾脏中分离条件永生化肾小球内皮细胞的方法。我们描述了使用磁珠、消化步骤、播种和培养 GEC-CD31 阳性从整个肾脏中分离肾小球的程序。

Tags

生物学,第187期,
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter