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Ex Vivo Analyse des transitoires Ca2+ activés mécaniquement dans les cellules urothéliales
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Ex Vivo Analyse des transitoires Ca2+ activés mécaniquement dans les cellules urothéliales

Article DOI: 10.3791/64532-v • 05:35 min • September 28th, 2022
September 28th, 2022 •
1,2, 1, 1,2
1Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, 2Department of Cell Biology, University of Pittsburgh

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Ce protocole décrit une méthodologie pour évaluer la fonction des canaux ioniques activés mécaniquement dans les cellules urothéliales natives à l’aide du capteur fluorescent Ca2+ GCaMP5G.

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Médecine Numéro 187 Mécanotransduction imagerie Ca2+ canaux ioniques stimulation mécanique GCaMP5G
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