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Journal / Biology / Visualizzazione della mitofagia con coloranti…
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Visualizzazione della mitofagia con coloranti fluorescenti per mitocondri e lisosomi
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Journal (Biology)
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
Journal (Biology)
Natural Transformation, Protein Expression, and Cryoconservation of the Filamentous Cyanobacterium Phormidium lacuna
 
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Visualizzazione della mitofagia con coloranti fluorescenti per mitocondri e lisosomi

Article DOI: 10.3791/64647-v • 07:56 min • November 30th, 2022
November 30th, 2022 •
1,2, 1, 3, 1, 1, 1,2
1Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, 2Key Laboratory of Laboratory Medicine, Ministry of Education, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, 3Department of Pharmacy, Shanghai East Hospital, Tongji University

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La mitofagia è il meccanismo primario del controllo di qualità mitocondriale. Tuttavia, la valutazione della mitofagia in vivo è ostacolata dalla mancanza di saggi quantitativi affidabili. Presentato qui è un protocollo per l'osservazione della mitofagia nelle cellule viventi utilizzando un colorante mitocondriale fluorescente verde e un colorante lisosoma rosso-fluorescente.

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Biologia Numero 189
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