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Immunology and Infection

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JoVE Journal Immunology and Infection
Rilevamento di cellule T polifunzionali in bambini vaccinati con vaccino contro l'encefalite giapponese tramite la tecnica della citometria a flusso
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Rilevamento di cellule T polifunzionali in bambini vaccinati con vaccino contro l'encefalite giapponese tramite la tecnica della citometria a flusso

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Il presente protocollo combina la stimolazione ex vivo e la citometria a flusso per analizzare i profili delle cellule T polifunzionali (T PF) nelle cellule mononucleate del sangue periferico (PBMC) all'interno di bambini vaccinati con virus dell'encefalite giapponese (JEV). Il metodo di rilevazione e lo schema di colori della citometria a flusso dei TPF specifici di JEV sono stati testati per fornire un riferimento per studi simili.

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Immunologia e infezione Numero 187 Cellule T polifunzionali encefalite giapponese vaccinazione citometria a flusso
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