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Ablação a Laser e Microscopia Intravital no Estudo do Remodelamento Intestinal
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Ablação a Laser e Microscopia Intravital no Estudo do Remodelamento Intestinal

Article DOI: 10.3791/64756-v • June 9th, 2023
June 9th, 2023 •
*1,2, *1,2, 3, 3, 1,2, 1,2
1Department of Molecular Pathology, The Netherlands Cancer Institute, 2Oncode Institute, 3Animal Laboratory Facility, The Netherlands Cancer Institute
* These authors contributed equally

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Apresentamos aqui um método para obter imagens do intestino após ferimento induzido por laser. Ao expor o intestino do camundongo a um laser multifóton, a perda de uma única ou múltiplas criptas é induzida localmente. Ao obter imagens repetidas da área danificada ao longo de meses, a dinâmica em tempo real da recuperação intestinal é capturada.

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Biologia Edição 196 ablação a laser microscopia intravital intestino regeneração dano remodelação de criptas
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