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Journal / Biochemistry / Immunfargingsbasert påvisning av dynamiske…
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Biochemistry

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JoVE Journal Biochemistry
Immunfargingsbasert påvisning av dynamiske endringer i røde blodcelleproteiner
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
Journal (Biochemistry)
Measurement of Ion Concentration in the Unstirred Boundary Layer with Open Patch-Clamp Pipette: Implications in Control of Ion Channels by Fluid Flow
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Journal (Biochemistry)
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
 
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Immunfargingsbasert påvisning av dynamiske endringer i røde blodcelleproteiner

Article DOI: 10.3791/64843-v • 10:07 min • March 17th, 2023
March 17th, 2023 •
1, 2
1Department of Molecular and Cellular Sports Medicine, German Sport University Cologne, 2Biorheology Research Laboratory, Menzies Health Institute, Griffith University Gold Coast

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Å fange dynamiske endringer i proteinaktiveringen av enucleated røde blodlegemer utgjør metodologiske utfordringer, som bevaring av dynamiske endringer i akutte stimuli for senere vurdering. Den presenterte protokollen beskriver prøvepreparering og fargeteknikker som muliggjør bevaring og analyse av relevante proteinendringer og påfølgende deteksjon.

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Biokjemi utgave 193 røde blodlegemer røde blodcelleproteiner mekanotransduksjon immunhistokjemi immunfluorescens nitrogenoksidsyntase
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