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Journal / Bioengineering / Undersøgelse af beige fedtbiologi og stofskifte…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Undersøgelse af beige fedtbiologi og stofskifte ved hjælp af CRISPR SunTag-p65-HSF1 aktiveringssystemet
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Undersøgelse af beige fedtbiologi og stofskifte ved hjælp af CRISPR SunTag-p65-HSF1 aktiveringssystemet

Article DOI: 10.3791/64849-v • 09:52 min • January 6th, 2023
January 6th, 2023 •
*1, *1, 1,2
1Obesity and Comorbidities Research Center, State University of Campinas, 2Department of Structural and Functional Biology, Institute of Biology, State University of Campinas
* These authors contributed equally

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Denne protokol præsenterer brugen af CRISPR SunTag-p65-HSF1 (SPH) i adipocytter (AdipoSPH) som en alternativ strategi til adeno-associeret virus (AAV) til undersøgelse af beige fedtbiologi. In vivo-injektion af AAV-bærende sgRNA rettet mod det endogene Prdm16-gen er tilstrækkeligt til at inducere beige fedtudvikling og forbedre det termogene genprogram.

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Denne måned i JoVE udgave 191 fedtvæv termogenese stofskifte CRISPR
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