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Journal / Bioengineering / Bioortogonal kemisk billeddannelse af…
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JoVE Journal Bioengineering
Bioortogonal kemisk billeddannelse af cellemetabolisme reguleret af aromatiske aminosyrer
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Journal (Bioengineering)
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Bioortogonal kemisk billeddannelse af cellemetabolisme reguleret af aromatiske aminosyrer

Article DOI: 10.3791/65121-v • 10:42 min • May 12th, 2023
May 12th, 2023 •
*1, *1, 1, 1, 1, 1
1Shu Chien-Gene Lay Department of Bioengineering, University of California San Diego
* These authors contributed equally

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Vi præsenterer en protokol til direkte visualisering af metaboliske aktiviteter i celler reguleret af aminosyrer ved hjælp af deuteriumoxid (tungt vand D2O) sondet stimuleret Raman-spredning (DO-SRS) mikroskopi, som er integreret med to-foton excitation fluorescens mikroskopi (2PEF).

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Bioengineering nr. 195
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