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Journal / Genetics / 인간 말초 림프구에서 γH2AX 및 53BP1의 이중…
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Genetics
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JoVE Journal Genetics
인간 말초 림프구에서 γH2AX 및 53BP1의 이중 면역형광
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인간 말초 림프구에서 γH2AX 및 53BP1의 이중 면역형광

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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이 프로토콜은 블레오마이신 처리된 인간 말초 림프구의 간기 핵에서 γH2AX 및 53BP1 병소의 동시 검출을 통해 DNA 이중 가닥 절단의 형성 및 복구를 평가하는 방법을 제시합니다.

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