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Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping
DOI:

07:03 min

November 09, 2020

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Chapters

  • 00:05Introduction
  • 00:41Hand‐Pollination in the Field
  • 01:45Hand Pollinations in the Laboratory
  • 02:34Microscopic Observations
  • 03:30Determining Incompatibility Relationships and Monitoring Flower Dates
  • 04:55Results: Determination of Pollination Requirements In Japanese Plum-Type Hybrids
  • 06:13Conclusion

Summary

Automatic Translation

A methodology for the determination of pollination requirements in Japanese plum-type hybrids is described, which combines field- and laboratory-pollinations and observations of pollen tubes under the fluorescence microscopy with the identification of S-genotypes by PCR and the monitoring of flowering for the selection of pollinizers.

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