Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography - JoVE

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Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography (Video) | JoVE

Published: December 15, 2023

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Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen, Thomas Lidahl³, Luiza Mendonça

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

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Keywords: Plunge Freezing Electron Microscopy Grids Cryotomography Gold Fiducials PBS Blotting Liquid Ethane Cryo-correlative Light And Electron Microscopy Cryo-electron Tomography U2OS Cells HIV Particles

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Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography. J. Vis. Exp. (Pending Publication), e200514, doi:10.3791/200514 (2023).

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