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Medicine
Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins (Video) | JoVE
Published: November 21, 2023
doi:
10.3791/200917
Hannes E. Villgrater*
1,2,3
,
Ruibing Xia*
1,2,3
,
Aparna Sharma Chivukula
2,3
,
Philipp Tomsits*
1,2,3
,
Sebastian Clauss*
1,2,3,4
1
University Hospital Munich, Department of Medicine I
,
Ludwig-Maximilians-University Munich (LMU)
,
2
Walter Brendel Center of Experimental Medicine (WBex)
,
LMU Munich
,
3
German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)
,
4
Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU)
,
LMU Munich
Tags
Immunofluorescence Staining
Myocardial Sleeves
Murine Pulmonary Veins
Cryomold
OCT Compound
Paraformaldehyde
PBS
Permeabilization
Blocking Buffer
Primary Antibody
Secondary Antibody
Hoechst 33342
Fluorescent Mounting Medium
check_url/200917?article_type=t
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Cite This Article
Tissue Embedding and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins.
J. Vis. Exp.
(Pending Publication), e200917, doi:10.3791/200917 (2023).
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