Michael A. Hough School of Life Sciences University of Essex Biography Publications Institution JoVE Articles Michael A. Hough has not added a biography. If you are Michael A. Hough and would like to personalize this page please email our Author Liaison for assistance. Publications Hydroxylamine Complexes of Cytochrome : Influence of Heme Iron Redox State on Kinetic and Spectroscopic Properties Inorganic Chemistry. Oct, 2020 | Pubmed ID: 32970420 Serial Femtosecond Zero Dose Crystallography Captures a Water-Free Distal Heme Site in a Dye-Decolorising Peroxidase to Reveal a Catalytic Role for an Arginine in Fe =O Formation Angewandte Chemie (International Ed. in English). 11, 2020 | Pubmed ID: 32780931 A Subtle Structural Change in the Distal Haem Pocket Has a Remarkable Effect on Tuning Hydrogen Peroxide Reactivity in Dye Decolourising Peroxidases from Streptomyces Lividans Dalton Transactions (Cambridge, England : 2003). Feb, 2020 | Pubmed ID: 31942590 Correction: One Fold, Two Functions: Cytochrome P460 and Cytochrome C'-β from the Methanotroph (Bath) Chemical Science. Sep, 2019 | Pubmed ID: 32055301 Successful Sample Preparation for Serial Crystallography Experiments Journal of Applied Crystallography. Dec, 2019 | Pubmed ID: 31798361 High-throughput Structures of Protein-ligand Complexes at Room Temperature Using Serial Femtosecond Crystallography IUCrJ. Nov, 2019 | Pubmed ID: 31709063 Dose-resolved Serial Synchrotron and XFEL Structures of Radiation-sensitive Metalloproteins IUCrJ. Jul, 2019 | Pubmed ID: 31316799 A Histidine Residue and a Tetranuclear Cuprous-thiolate Cluster Dominate the Copper Loading Landscape of a Copper Storage Protein from Streptomyces Lividans Chemistry (Weinheim an Der Bergstrasse, Germany). Aug, 2019 | Pubmed ID: 31111982 Correction: One Fold, Two Functions: Cytochrome P460 and Cytochrome C'-β from the Methanotroph (Bath) Chemical Science. 03, 2019 | Pubmed ID: 30997901 One Fold, Two Functions: Cytochrome P460 and Cytochrome '-β from the Methanotroph (Bath) Chemical Science. 03, 2019 | Pubmed ID: 30996884 An Aromatic Dyad Motif in Dye Decolourising Peroxidases Has Implications for Free Radical Formation and Catalysis Chemistry (Weinheim an Der Bergstrasse, Germany). Apr, 2019 | Pubmed ID: 30945782 Resolving Polymorphs and Radiation-driven Effects in Microcrystals Using Fixed-target Serial Synchrotron Crystallography Acta Crystallographica. Section D, Structural Biology. Feb, 2019 | Pubmed ID: 30821704 A QM/MM Study of Nitrite Binding Modes in a Three-Domain Heme-Cu Nitrite Reductase Molecules (Basel, Switzerland). Nov, 2018 | Pubmed ID: 30453538 Enzyme Catalysis Captured Using Multiple Structures from One Crystal at Varying Temperatures IUCrJ. May, 2018 | Pubmed ID: 29755744 From Crystal to Structure with CCP4 Acta Crystallographica. Section D, Structural Biology. Feb, 2018 | Pubmed ID: 29533232 A Cytosolic Copper Storage Protein Provides a Second Level of Copper Tolerance in Streptomyces Lividans Metallomics : Integrated Biometal Science. 01, 2018 | Pubmed ID: 29292456 Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome C Increases Its Peroxidatic Activity Biochemistry. 11, 2017 | Pubmed ID: 29083920 Distinguishing Nitro Vs Nitrito Coordination in Cytochrome C' Using Vibrational Spectroscopy and Density Functional Theory Inorganic Chemistry. Nov, 2017 | Pubmed ID: 29053273 Active-site Protein Dynamics and Solvent Accessibility in Native Copper Nitrite Reductase IUCrJ. Jul, 2017 | Pubmed ID: 28875036 Recent Structural Insights into the Function of Copper Nitrite Reductases Metallomics : Integrated Biometal Science. 11, 2017 | Pubmed ID: 28702572 Choosing the Optimal Spectroscopic Toolkit to Understand Protein Function Bioscience Reports. Jun, 2017 | Pubmed ID: 28522552 Photoreduction and Validation of Haem-ligand Intermediate States in Protein Crystals by Single-crystal Spectroscopy and Diffraction IUCrJ. May, 2017 | Pubmed ID: 28512573 Engineering Proximal Distal Heme-NO Coordination Dinitrosyl Dynamics: Implications for NO Sensor Design Chemical Science. Mar, 2017 | Pubmed ID: 28451315 Interaction of Azole-Based Environmental Pollutants with the Coelomic Hemoglobin from Amphitrite Ornata: A Molecular Basis for Toxicity Biochemistry. 05, 2017 | Pubmed ID: 28387506 Active-site Maturation and Activity of the Copper-radical Oxidase GlxA Are Governed by a Tryptophan Residue The Biochemical Journal. 02, 2017 | Pubmed ID: 28093470 Serial Crystallography Captures Enzyme Catalysis in Copper Nitrite Reductase at Atomic Resolution from One Crystal IUCrJ. Jul, 2016 | Pubmed ID: 27437114 Heterogeneity in the Histidine-brace Copper Coordination Sphere in Auxiliary Activity Family 10 (AA10) Lytic Polysaccharide Monooxygenases The Journal of Biological Chemistry. Jun, 2016 | Pubmed ID: 27129229 Cytochromes C': Structure, Reactivity and Relevance to Haem-Based Gas Sensing Advances in Microbial Physiology. 2015 | Pubmed ID: 26616515 Structural Insights into Conformational Switching in the Copper Metalloregulator CsoR from Streptomyces Lividans Acta Crystallographica. Section D, Biological Crystallography. Sep, 2015 | Pubmed ID: 26327377 GlxA is a New Structural Member of the Radical Copper Oxidase Family and is Required for Glycan Deposition at Hyphal Tips and Morphogenesis of Streptomyces Lividans The Biochemical Journal. Aug, 2015 | Pubmed ID: 26205496 Resonance Raman Spectra of Five-Coordinate Heme-Nitrosyl Cytochromes C': Effect of the Proximal Heme-NO Environment Biochemistry. Jun, 2015 | Pubmed ID: 25961377 Conformational Control of the Binding of Diatomic Gases to Cytochrome C' Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry. Jun, 2015 | Pubmed ID: 25792378 Antiandrogens Act As Selective Androgen Receptor Modulators at the Proteome Level in Prostate Cancer Cells Molecular & Cellular Proteomics : MCP. May, 2015 | Pubmed ID: 25693800 Challenges and Solutions for the Analysis of in Situ, in Crystallo Micro-spectrophotometric Data Acta Crystallographica. Section D, Biological Crystallography. Jan, 2015 | Pubmed ID: 25615857 NO Binding to the Proapoptotic Cytochrome C-cardiolipin Complex Vitamins and Hormones. 2014 | Pubmed ID: 25189388 The Structure of a Class 3 Nonsymbiotic Plant Haemoglobin from Arabidopsis Thaliana Reveals a Novel N-terminal Helical Extension Acta Crystallographica. Section D, Biological Crystallography. May, 2014 | Pubmed ID: 24816109 Fingerprinting Redox and Ligand States in Haemprotein Crystal Structures Using Resonance Raman Spectroscopy Acta Crystallographica. Section D, Biological Crystallography. May, 2014 | Pubmed ID: 24816098 The Benefit of the European User Community from Transnational Access to National Radiation Facilities Journal of Synchrotron Radiation. May, 2014 | Pubmed ID: 24763656 Structural and Mechanistic Insights into an Extracytoplasmic Copper Trafficking Pathway in Streptomyces Lividans The Biochemical Journal. May, 2014 | Pubmed ID: 24548299 The Hydrogen-peroxide-induced Radical Behaviour in Human Cytochrome C-phospholipid Complexes: Implications for the Enhanced Pro-apoptotic Activity of the G41S Mutant The Biochemical Journal. Dec, 2013 | Pubmed ID: 24099549 Response to Copper Stress in Streptomyces Lividans Extends Beyond Genes Under Direct Control of a Copper-sensitive Operon Repressor Protein (CsoR) The Journal of Biological Chemistry. May, 2012 | Pubmed ID: 22451651 Fixed Target Serial Data Collection at Diamond Light Source Sam Horrell1, Danny Axford1, Nicholas E. Devenish1, Ali Ebrahim1, Michael A. Hough2, Darren A. Sherrell1,3, Selina L. S. Storm1,4, Ivo Tews5, Jonathan A. R. Worrall2, Robin L. Owen1 1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences, University of Essex, 3X-ray Science Division, Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences, University of Southampton JoVE 62200 Biochemistry
Fixed Target Serial Data Collection at Diamond Light Source Sam Horrell1, Danny Axford1, Nicholas E. Devenish1, Ali Ebrahim1, Michael A. Hough2, Darren A. Sherrell1,3, Selina L. S. Storm1,4, Ivo Tews5, Jonathan A. R. Worrall2, Robin L. Owen1 1Diamond Light Source, Harwell Science and Innovation Campus, 2School of Life Sciences, University of Essex, 3X-ray Science Division, Argonne National Laboratory, 4European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, 5Biological Sciences, Institute for Life Sciences, University of Southampton JoVE 62200 Biochemistry