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Reprogramming PDAC and Pancreatic Cells into Induced Pluripotent Stem Cells

February 2nd, 2024
1,2, 3, 4, 4,5, 4,6, 3,6, 1
1Centre for Regenerative Medicine, Institute of Regeneration and Repair, Institute of Stem Cell Research, The University of Edinburgh, 2King Abdulaziz City for Science and Technology Health Sector (KACST), 3Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health & Science University, 4Brenden-Colson Canter for Pancreatic Care, Oregon Health & Science University, 5Department of Surgery, Oregon Health & Science University, 6Department of Molecular & Medical Genetics, Oregon Health & Science University

Summary
Automatic Translation

Das vorliegende Protokoll beschreibt die Reprogrammierung von duktalen Adenokarzinomen der Bauchspeicheldrüse (PDAC) und normalen duktalen Epithelzellen der Bauchspeicheldrüse in induzierte pluripotente Stammzellen (iPSCs). Wir bieten ein optimiertes und detailliertes Schritt-für-Schritt-Verfahren, von der Vorbereitung des Lentivirus bis zur Etablierung stabiler iPSC-Linien.

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