Monitoring Epileptiform Events in Drosophila via Calcium Imaging

October 13th, 2023

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Ming-Feng He¹, Chu-Qiao Liu², Xi-Xing Zhang², Yong-Miao Lin², Yu-Ling Mao^3,4, Jing-Da Qiao¹

¹Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, ²The Second Clinical Medicine School of Guangzhou Medical University, ³Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, ⁴Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

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هنا ، نقدم بروتوكولا لتصوير الكالسيوم خارج الجسم الحي في ذبابة الفاكهة البالغة التي تعبر عن GCaMP6 لمراقبة أنشطة الصرع. يوفر البروتوكول أداة قيمة للتحقيق في أحداث ictal في ذبابة الفاكهة للبالغين من خلال تصوير الكالسيوم خارج الجسم الحي ، مما يسمح باستكشاف الآليات المحتملة للصرع على المستويات الخلوية.

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Monitoring Epileptiform Events in Drosophila via Calcium Imaging

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