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Monitoring Epileptiform Events in Drosophila via Calcium Imaging

October 13th, 2023
1, 2, 2, 2, 3,4, 1
1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University

Summary
Automatic Translation

Her præsenterer vi en protokol for ex vivo calciumbilleddannelse i GCaMP6-ekspresserende voksen Drosophila til overvågning af epileptiforme aktiviteter. Protokollen giver et værdifuldt værktøj til undersøgelse af ictale hændelser hos voksne Drosophila gennem ex vivo calciumbilleddannelse, hvilket giver mulighed for udforskning af de potentielle mekanismer for epilepsi på celleniveau.

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