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Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems

January 05, 2024
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Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

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Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

01:17 min
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Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

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1Department of Microbiology,University of Manitoba

Summary

Automatic Translation

We describe a facile procedure for the single-copy chromosomal complementation of an efflux pump gene using a mini-Tn7-based expression system into an engineered efflux-deficient strain of Acinetobacter baumannii. This precise genetic tool allows for controlled gene expression, which is key for the characterization of efflux pumps in multidrug resistant pathogens.

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