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Immunology and Infection

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Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems

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Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems

DOI:

10.3791/201441-v

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01:17 min

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January 05, 2024

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Dawn White, Ayush Kumar

¹Department of Microbiology,University of Manitoba

Tags

Keywords: Electrocompetent Cells Single-copy Gene Expression Efflux-deficient Bacterial Strain Cell Preparation Centrifugation Cell Pellet Sterile Water Cell Suspension Microfuge Tubes Electroporation Cuvettes

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