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Journal
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Immunology and Infection
/
Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems
/
Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems
DOI:
10.3791/201442-v
•
03:04 min
•
January 05, 2024
•
Dawn White
,
Ayush Kumar
1
Department of Microbiology
,
University of Manitoba
Tags
Electroporation
PTNS2 Helper Plasmid
PUC18-Tmini-Tn7-Tlac Gentamicin AdeIJK Insertion Plasmid
Electrocompetent Cells
LB Broth
LB Agar
Gentamicin
Colony PCR
ABglmS2 Forward
Tn7 Reverse
A.baumannii ATCC 17978
AB 258
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